MOLECULAR BIOLOGY CORE SERVICES
The purpose of the UMMS Center for AIDS Research (CFAR) Molecular Biology Core (MBC) is to serve the research needs of multiple, independent investigators conducting HIV/AIDS research at the University of Massachusetts Medical School (UMMS). Based on the NIH CFAR Program goals and objectives, Core E provides essential molecular biology reagents, services, facilities, training, and technical expertise in support of basic and clinical HIV/AIDS research programs. Core E also supports technology development, educational initiatives, and outreach activities related to HIV/AIDS.
The UMMS CFAR MBC offers users a variety of molecular biology reagents and analytical services at highly competitive prices.
- Discounted Oligonucleotides: DNA, RNA, Modifications, siRNA duplexes
http://www.umassmed.edu/nemo/oligo/ - Standard DNA Sequence Analysis
http://www.umassmed.edu/MBCL - DNA Fragment Analysis (e.g. Genotyping, AFLP, LOH, SNP)
http://www.umassmed.edu/MBCL - Deep Sequencing
http://www.umassmed.edu/nemo - Discounted Reagent Program: Applied Biosystems, Ambion, and IDT
http://www.umassmed.edu/nemo/mbcl/mbcl-reagent-program/ - Plasmid, Primer, and Cell Line Repositories
- Molecular Biology Technical Resources and Training
- Real-Time PCR Analysis Service
Additional Resources
HIV Sequence Database
This site contains many useful components, including Reviews from the Human Retroviruses and AIDS Compendia; access to DNA, cDNA, and protein sequences from HIV and SIV, as well as analysis programs; tools and tutorials for the construction of evolutionary trees; and information on geographic distribution of isotypes.
http://hiv-web.lanl.gov/
HIV Database at Stanford
A curated database of RT and protease sequences -- an excellent resource for researchers studying the molecular bases for the evolution of antiretroviral drug resistance.
http://hivdb.stanford.edu/hiv/
AIDS Reagent Program
Catalog of available reagents, application forms, online ordering, and instructions for submission of reagents.
http://www.aidsreagent.org/ (Reagent Program Main Page)
http://www.niaid.nih.gov/reposit/tetramer/index.html (Tetramer facility)
UMass Bioinformatics Tools
Primer Selection, BioNetbook, Restriction Mapping, Database Searches, etc.
http://biotools.umassmed.edu
National Center for Biotechnology Information
All your favorites: Genbank, PUBMED, OMIM, Entrez, Genome Project, etc.
http://www.ncbi.nlm.nih.gov/
Next Generation ~ Deep Sequencing resources
http://biotools.umassmed.edu/BioCore/nextgen/
http://wiki.umassmed.edu/rc/wiki/index.php/IlluminaPipeline
DeepSeq FAQ http://www.umassmed.edu/nemo/deep-sequence-FAQ/